Whether or not they can be grown under "artificial" conditions, however, remains to be seen. Most saprophytic fungi, especially primary saprophytes, are easy to isolate from nature. I SI jjjo hen a mushroom is brought into culture from ths^/iif little is known about its performance until trials are conducted. * 1/20 of a gram of gentamycin sulfate per liter of media sufficiently inhibits bacteria to a containable level. Agrocybe aegerita Flammulina velutipes Ganoderma lucidum Hericium erinaceus Hypsizygus tessulatus Hypsizygus ulmarius Lentinula edodes Pholiota nameko Pleurotus citrinopileatus Pleurotus djamor complex Pleurotus euosmus Pleurotus ostreatus Pleurotus djamor Pleurotus pulmonarius Those which do benefit from disturbance are excellent candidates for liquid inoculation techniques. Those species in bold lettering are known by this author to benefit from the timely disturbance of primordia. *The formation of primordia on this medium is often strain specific. The following list of species are those that produce volunteer primordia on 2% enriched malt extract agar, supplemented with. With most strains however, the damaged primordia revert to vegetative growth. Several mushroom species have mycelial networks which, when they are disturbed at primordia formation, result in a quantum leap in the vigor of growth and in the number of subsequently forming primordia. As a group, those strains needing bacteria to fruit do not form primordia on sterile media. After performance standards are ascertained, the second approach is to alter the media, specifically improving and designing its composition for the species selected. The first is to devise a standard mei lia, a background against which all strains and species can be compared. Two approaches lead to primordia formation from cultured mycelium. Integral to the success of the Mushroom Life Cycle is the mycelial path leading to primordia formation.To this end, the mushroom and the cultivator share common interests.The occurrence of primordia not only is a welcome affirmation of the strain's identity but is also indicative of its readiness to fruit Hence, I tend to favor strains which voluntarily form primordia. The cultivator plays an active role in developing strains by physically selecting those which look "good". Every cultivator contributes to the body of knowledge each time a mushroom is cultured and studied. In this chapter, attention and insights are given for many species. The voluntary and spontaneous formation of miniature mushrooms in a petri dish is a delightful experience for all cultivators.
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